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Professor W. Grimm (Stavropol State Medical University) and his colleagues Professor T. Dittmar (University of Witten-Herdecke, Germany) and Assistant Professor D. Wieder (University of Bielefeld, Germany), using on-line conference connection from stem cell laboratory of StSMU, have grown 13 samples criofrozed stem cells.



The research team from Department of Pathology and Microbiology of StSMU took part in this project either.
To minimize the risk of contamination by mycoplasma the method of storage without liquid nitrogen was used.


As a cooling agent culture medium 199 with the indicator of the cell cultures and extract of Lucerna, increasing the proliferative activity of stem cells derived from the human palate, and improving surviving of cell cultures was used.
After four days the stem cells derived from the human palate developed into cell spheroids after which the research team collected the necessary number of cells by centrifugation.

During the experiment, researchers added as a cooling agent DMEM / F12 without L-glutamine supplemented with B27 and 10% dimethyl sulfoxide solution, and the mix was placed in a sealed container for 4 hours at a temperature of -20 ° C. Then the cells in cooling agent were frozen to a temperature of -80 ° C in laboratory center of Stavropol Research Anti-Plague Institute.
Located in the deep freezing state the stem cells derived from human palate, will be used in subsequent experiments laboratory cell technologies of StSMU.

Dr. Wolf-Dieter Grimm, Professor of Periodontology, University of Witten/Herdecke, Germany, Stavropol State Medical University, Russian Federation participate in 2014 in follow conferences:

1. BIT's 7th Annual World Congress of Regenerative Medicine & Stem Cells-2014

Theme: Translation from Concepts to Market

Time: November 13-16, 2014, Venue: Haikou,China

2. BIT’s 5th World Gene Convention-2014 
Theme: Olympic Campaign of New Biotechnology

Time: November 13-16, 2014
Venue: Hainan International Convention and Exhibition Center, Haikou, China

3. 1st Annual International Congress of Oral & Dental Medicine, November 13-16, 2014 in Haikou, China

Title: Photodynamic Treatment (PDT)- Comparative Study Between Two Different Modalities of Conservative Periodontal Therapy.

4. Teilnahme als eingeladener Referent am Symposion „Realistische Einstiegskonzepte in die Implantologie: Chirurgie, Prothetik, Nachsorge – praxisnah vermittelt”

5. Master Classes in Advanced Periodontology and Implantology -Minimal-invasive Periodontology and Implantology-State of the Art-

Organized and presented by Amfodent, St. Petersburg/Russia