International Science in University
Professor W. Grimm (Stavropol State Medical University) and his colleagues Professor T. Dittmar (University of Witten-Herdecke, Germany) and Assistant Professor D. Wieder (University of Bielefeld, Germany), using on-line conference connection from stem cell laboratory of StSMU, have grown 13 samples criofrozed stem cells.
The research team from Department of Pathology and Microbiology of StSMU took part in this project either.
To minimize the risk of contamination by mycoplasma the method of storage without liquid nitrogen was used.
As a cooling agent culture medium 199 with the indicator of the cell cultures and extract of Lucerna, increasing the proliferative activity of stem cells derived from the human palate, and improving surviving of cell cultures was used.
After four days the stem cells derived from the human palate developed into cell spheroids after which the research team collected the necessary number of cells by centrifugation.